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Cloning and Expression
Enzymes
BamHI
Supplier Images
BamHI
BamHI from New England Biolabs
Be the first to write a review!
Citations:
(22)
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New England Biolabs for
BamHI
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Description
BamHI
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BamHI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0136T
Quantity
10000 units
Concentration
100,000 units/ml
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
5'-G/GATCC-3'
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (22)
(1)
Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants
Cell Reports
May 30, 2023
Jianbo Wu, Zhenguo Chen, Yidan Gao, Zegen Wang, Jiarong Wang, Bing-Yu Chiang, Yunjiao Zhou, Yuru Han, Wuqiang Zhan, Minxiang Xie,
et al.
,
New England BioLabs
,Cat#
R3552L
BsiwI-HF
,
New England BioLabs
,Cat#
R3553L
BamH1,
New England BioLabs
,Cat#
R0136M
XhoI
,
New England BioLabs
,Cat#
R0146L
SalI-HF
,
New England BioLabs
,Cat#
R3138S
10× NEB buffer 2,
New England BioLabs
(2)
Functional analysis of the short splicing variant encoded by CHI3L1/YKL-40 in glioblastoma
Front Oncol
November 2, 2022
Mengqi Shi, Qianyun Ge, Xinrong Wang, Wenbin Diao, Ben Yang, Sipeng Sun, Guohui Wang, Tian Liu, Andrew Man-Lok Chan, Zhiqin Gao,
et al.
purified by a gel extraction kit (Geneaid, DF300). Purified DNA fragments were digested with
BamHI
(
NEB
, #
R0136T
) and
EcoRI
(
NEB
, #
R0101T
) and cloned into the pBABE-puro and pBABE-Neo (Addgene, #1767) vectors using
(3)
Probing DNA-protein interactions using single-molecule diffusivity contrast
Biophysical Reports
September 8, 2021
Hugh Wilson, Miles Lee, Quan Wang
Restriction enzymes (
BamHI
and
EcoRI
) were purchased from
New England BioLabs
(Ipswich, MA) (
BamHI
:
R0136M
,
EcoRI
:
R0101M
) and used without further
purification
. The stock concentrations of the enzymes (
BamHI
:
(4)
Systematic dissection of σ70 sequence diversity and function in bacteria
Cell Reports
August 24, 2021
Jimin Park, Harris H. Wang
Coulter,
A63881
). Purified library and pNJ7 plasmid (Johns et al., 2018) were digested with
BamHI
(
NEB
,
R0136M
) and
PstI
(
NEB
,
R0140M
),
PCR
purified (Zymo, D4033), and ligated with
T4 DNA ligase
(
NEB
,
M0202M
). Resulting
(5)
An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
STAR protocols
March 19, 2021
Haque M, Xiong X, Lei F, Das JK, Song J.
TCR genes,Dr. Dario A.A. Vignali (University of Pittsburgh),N/A BamH1/EcoR1,
New England Biolabs
,CAT#
R0136T
/
R0101T
EcoRI
/
BglII
,
New England Biolabs
,CAT#
R0101T
/
R0144M
,, "Buffer, media, and reagents","Buffer,
(6)
Fluorescent Protein Variants Generated by Reassembly between Skeleton and Chromophore
ACS Omega
February 2, 2021
Tingting Sun, Tianpeng Li, Ke Yi, Guoquan Yan, Xiaolian Gao
sequences were inserted into the pET28(+) plasmid through sequential Xho I (R0146V, NEB) and BamH I (
R0136T
,
NEB
) digestion, followed by
ligation
with T4 ligase (
M0202L
,
NEB
). Then, the product was expressed
(7)
Cyclic Peptide Mimotopes for the Detection of Serum Anti-ATIC Autoantibody Biomarker in Hepato-Cellular Carcinoma
International journal of molecular sciences
December 19, 2020
Heo CK, Hwang HM, Lim WH, Lee HJ, Yoo JS, Lim KJ, Cho EW.
(8)
Regulation of Mother-to-Offspring Transmission of mtDNA Heteroplasmy
Cell metabolism
December 3, 2019
Latorre-Pellicer A, Lechuga-Vieco AV, Johnston IG, Hämäläinen RH, Pellico J, Justo-Méndez R, Fernández-Toro JM, Clavería C, Guaras A, Sierra R,
et al
.
Recombinant Proteins" ,, Φ29 DNA polymerase,
New England Biolabs
,
M0269L
BamH1,
New England Biolabs
,
R0136M
SspI
,
New England Biolabs
,
R0132S
Lambda Exonuclease
.,
New England Biolabs
,
M0262S
T4 DNA ligase
,
New England Biolabs
(9)
Structural Basis of the Differential Binding of Engineered Knottins to Integrins αVβ3 and α5β1
Structure
September 1, 2019
Johannes F. Van Agthoven et al.
2009b)",N/A Peptide 2.5D,"(Kimura et al., 2009b)",N/A
KpnI
,
New England Biolabs
,
R0142M
BamhI
,
New England Biolabs
,
R0136M
EcoRI
,
New England Biolabs
,
R0101M
BssHII
,
New England Biolabs
,
R0199M
XbaI
,
New England Biolabs
,
R0145M
Penicillin-Streptomycin,
ThermoFisher
,
15140122
PBS,Boston
(10)
Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae
Genes (Basel)
May 22, 2019
Lance E. Keller, Anne-Stéphanie Rueff, Jun Kurushima, Jan-Willem Veening
(11)
Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
Scientific Reports
December 1, 2018
Luis Alfonso Yañez-Guerra, Jérôme Delroisse, Antón Barreiro-Iglesias, Susan E. Slade, James H. Scrivens, Maurice R. Elphick
containing the ArLQ precursor cDNA was linearised using the restriction enzymes BamH1 (
NEB
; Cat. No.
R0136T
) for the antisense probe and Xho1 (
NEB
; Cat. No.
R0146S
) for the sense probe. The linearised plasmids
(12)
SYK kinase mediates brown fat differentiation and activation
Nature communications
December 13, 2017
Knoll M, Winther S, Natarajan A, Yang H, Jiang M, Thiru P, Shahsafaei A, Chavarria TE, Lamming DW, Sun L,
et al
.
in Supplementary Table 2.
Southern blotting
10 µg genomic BAT DNA were digested with 200 U
BamHI
(#
R0136M
,
NEB
) for 16 h at 37 °C and separated with a 0.7% agarose gel for 24 h at 80 V at 4 °C. Following acid-base
(13)
Double mimicry evades tRNA synthetase editing by toxic vegetable-sourced non-proteinogenic amino acid
Nature Communications
December 1, 2017
Youngzee Song et al.
(14)
Mettl3-mediated m6A regulates spermatogonial differentiation and meiosis initiation
Cell Research
September 1, 2017
Kai Xu et al.
Thus, purified cDNA was circularized with Circligase II (Epicentre, CL9021K) and re-linearized with
BamHI
(
NEB
,
R0136M
). Libraries were
PCR
amplified with Accuprime Supermix 1 enzyme for 18 cycles and size-selected on an
(15)
Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesis
eLife
September 13, 2016
Walentek P, Quigley IK, Sun DI, Sajjan UK, Kintner C, Harland RM.
Walentek et al., 2013).
Subcloning
was performed using BamH1 and EcoR1 (
New England Biolabs
, #
R0101T
; #
R0136T
) restriction enzymes and the following primers (shown 5' to 3'):
Cp110
forward AAAAAAGGATCC ATGGAGCCATATGAAGAATTTTATAAG;
Cp110
(16)
PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis
Nature Communications
December 8, 2015
Christian F. Nielsen et al.
(17)
Modulation of RNA Polymerase II Phosphorylation Downstream of Pathogen Perception Orchestrates Plant Immunity
Cell Host & Microbe
December 10, 2014
Fangjun Li et al.
(18)
A genome-wide inducible phenotypic screen identifies antisense RNA constructs silencing Escherichia coli essential genes
FEMS Microbiology Letters
April 1, 2012
Jia Meng, Gregory Kanzaki, Diane Meas, Christopher K. Lam, Heather Crummer, Justina Tain, H. Howard Xu
(19)
Tracing the Domestication of a Biofilm-Forming Bacterium
Journal of Bacteriology
April 15, 2011
Anna L McLoon, Sarah B Guttenplan, Daniel B Kearns, Roberto Kolter, Richard Losick
(20)
Disruption of genomic neighbourhood at the imprinted IGF2-H19 locus in Beckwith-Wiedemann syndrome and Silver-Russell syndrome
Human molecular genetics
April 1, 2011
Raffaella Nativio, Angela Sparago, Yoko Ito, Rosanna Weksberg, Andrea Riccio, Adele Murrell
by
centrifugation
at 3500 rpm for 5 min. 1.5 × 106 nuclei were resuspended in 300 μl of 1X Buffer 3 [
R0136M
,
New England Biolabs
(NEB) with added
protease inhibitors
(P8340, Sigma) and 1.8%
Triton X-100
(T8787,
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BamHI from New England Biolabs
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